ABSTRACT
Objective To explore the application value of ATP bioluminescence in assaying the cleaning quality of uterine suction.Methods According to the principle of random,we selected 120 uterine suction tube after use.60 used tubes in A group were given water immersion + high-pressure water jets wash + dry method,60 used tubes in B group were given alkaline cleaning solution soak + ultrasonic + high-pressure water washing + manual scrub + drying method.The quality of suction tube was initially tested with the visual method after cleaning,Straw inside the top cavity,2.5cm at inside straw joint and the front of straw outer wall,10cm at suction cavity wall,before and after cleaning the residues of bacteria were assayed with the ATP bioluminescence and bacteria counting method for parallel comparison.Results After cleaning drying program,the RLU of two groups in suction head internal top,2.5cm at inside straw joint,the front of straw outer wall,10cm at suction cavity wall had significant differences (t =26.81,29.13,7.14,55.74,P ＜ 0.01).The cleaning effect of B group was better than A group..Bacteria counting method was used to detect pathogenic microorganism that was not detected.The qualified rate of cleaning at four parts was as following:group A:70.0％,85.0％,93.3％,93.3％.B group:95.0％,98.3％,100.0％,100.0％,and the qualified rate of group B was significantly higher than group A (x2 =12.99,6.98,4.14,4.14,all P ＜ 0.05).Conclusion ATP bioluminescence method can dynamically rapid detect the bacteria before and after intrauterine suction cleaning residues,can be viewed as an effective method to detect quality of uterine suction cleaning.
ABSTRACT
OBJECTIVE To observe the effect of flushing dental handpieces to prevent suction-induced contamination and to lower the bacterial level in dental unit waterlines,and then to analyze the time-effect relationship of flushing.METHODS Twelve BienAir handpieces(group A) and 12 W&H TA-96 handpieces(group B) were employed in this study.The water samples from each handpiece′s outlet were immediately taken once when operations of de-caries,cavity-preparing and dental-drilling had been completed,and then taken once per 0.5 min while the handpieces were being flushed by running without work for 4 min.The bacterial colony formation of these water samples was counted on R2A agar plates.Colony forming units vs flushing time were then compared.RESULTS Alike in groups A and B,water bacterial levels were lowered the most significantly while flushing the handpieces for 0.5 min.BienAir or W&H TA-96 handpieces still showed decreased levels of water bacteria when being flushed for 3 or 2.5 min respectively.Afterwards,the flushing effect reached to a platform,that was,more flushing time didn′t bring the bacterial level down further.CONCLUSIONS Flushing handpieces by running without work can significantly reduce the level of bacterial contamination in the waterlines.Different types of handpieces may have different flushing time at which the most effect is reached.